Modulation of Splicing Events in Histone Deacetylase 3 by Various Extracellular and Signal Transduction Pathways

Regulation of alternative splicing by signal transduction pathways.

Alternative splicing is now recognized as a ubiquitous mechanism for controlling gene expression in a tissue-specific manner. A growing body of work from the past few years as begun to also highlight the existence of networks of signal-responsive alternative splicing in a variety of cell types. While the mechanisms by which signal transduction pathways influence the splicing machinery are relat...

Modulation of neuronal signal transduction systems by extracellular ATP.

The secretion of ATP by stimulated nerves is well documented. Following repetitive stimulation, extracellular ATP at the synapse can accumulate to levels estimated to be well over 100 microM. The present study examined the effects of extracellular ATP in the concentration range of 0.1-1.0 mM on second-messenger-generating systems in cultured neural cells of the clones NG108-15 and N1E-115. Cell...

Signal transduction pathways for activation of extracellular signal-regulated kinase by arachidonic acid in rat neutrophils.

Phosphorylation of extracellular signal-regulated kinase (ERK) in response to arachidonic acid (AA) was rapid and transient, peaking at 1 min and disappearing after 3 min, and it was accompanied by an increase in ERK activity in rat neutrophils. We examined the upstream regulation of AA-stimulated ERK activation using one of the following signaling pathway inhibitors to pretreat rat cells: the ...

HDAC3 (histone deacetylase 3)

Histone Deacetylase Activity Modulates Alternative Splicing

There is increasing evidence to suggest that splicing decisions are largely made when the nascent RNA is still associated with chromatin. Here we demonstrate that activity of histone deacetylases (HDACs) influences splice site selection. Using splicing-sensitive microarrays, we identified ∼700 genes whose splicing was altered after HDAC inhibition. We provided evidence that HDAC inhibition indu...